GeNext Genomics

  The goal is to generate an extensive Antibody Library of phage-displayed human scFV with a 6.7 * 109 member count that could serve the application in the respective industries and research laboratories. The Antibody Library is to be generated with improvising steps in library construction. Fourteen different protein antigens were under the affinity to select antibodies from the library. The goal is the generation of reagents from these large libraries that study the function of gene products identified by the genome projects. Methods Construction of the VH-VL Library. Total RNA is prepared when different samples are taken from human spleen cells and human peripheral blood lymphocytes. The cDNA synthesised using the total RNA is primed with Human lgG Forward and Reverse primer. This VH-VL gene repertoire is amplified from  cDNA of Lymphocyte and cloned in Phagemid vector. The resulting antibody gene library is transformed in E.coli TG1 cells and Phages are prepared to displ...

What are MAbs made of? An antibody is a protein that sticks to a specific protein called an antigen. Antibody plays a role circulating throughout the body until it finds the antigen target.  Monoclonal antibodies are the antibodies that target an antigen, a cancer cell antigen, explicitly. These can be made in laboratories and are said to be monoclonal antibody drugs .  These mAbs play a vital role in treating several diseases that include some types of cancers. Monoclonal antibody drugs can be prepared with researchers at work and proven effective against most treatments, including some cancers. The mAbs are man-made proteins that are similar to human antibodies that form the immune system. There are four ways to make mAbs, and are named on the particular ones as follows:  Murine These proteins are made via mouse proteins and the names of the treatment associated with this mAb end in -omab. Chimeric These proteins combine part mouse and part human, with treatment names e...

  Tuberculosis, caused by Mycobacterium tuberculosis , is a severe disease that mainly affects the lungs. TB remains the deadliest killer disease despite the current epidemic of COVID.  Like COVID, Tuberculosis bacteria can spread from person to person through sneezing and coughing And post COVID, and the Corona Virus has also posed a risk to people with TB. TB is a potentially fatal disease and has many causes, yet it is preventable and has a wide range of treatments in the market. When the TB affects the lungs, the disease results in being more contagious, with the person usually getting sick and close contact with them can make others affected too. Hence, it also brings isolation and social stigma to the patient suffering from TB.  It is mainly considered the disease of the poor as it is prevalent in a more crowded area with fewer resources. TB remains with us in a different form. TB Infection (Latent TB):  An individual with TB bacteria in the body who shows no s...