The goal is to generate an extensive Antibody Library of phage-displayed human scFV with a 6.7 * 109 member count that could serve the application in the respective industries and research laboratories. The Antibody Library is to be generated with improvising steps in library construction. Fourteen different protein antigens were under the affinity to select antibodies from the library. The goal is the generation of reagents from these large libraries that study the function of gene products identified by the genome projects. Methods Construction of the VH-VL Library. Total RNA is prepared when different samples are taken from human spleen cells and human peripheral blood lymphocytes. The cDNA synthesised using the total RNA is primed with Human lgG Forward and Reverse primer. This VH-VL gene repertoire is amplified from cDNA of Lymphocyte and cloned in Phagemid vector. The resulting antibody gene library is transformed in E.coli TG1 cells and Phages are prepared to displ...